Human 14-3-3η (3C-Cleaved)

Protein Information

Description

Recombinant human 14-3-3η protein post 3C cleavage, originally tagged with GST, His8, and Avi.

Molecular Weight

Un-cleaved: 58.93 kDa / Cleaved: 32.49 kDa

pI

Un-cleaved: 5.31 / Cleaved: 5.05

Expression System

BL21(DE3)

Buffer

20 mM HEPES (pH 8.0 at 4°C), 150 mM NaCl, 0.5 mM TCEP

Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPN

LPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRI

AYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVV

LYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDH

PPKGSSLEVLFQGPHHHHHHHHGSSGLNDIFEAQKIEWHEGSSENLYFQSGD

REQLLQRARLAEQAERYDDMASAMKAVTELNEPLSNEDRNLLSVAYKNVVG

ARRSSWRVISSIEQKTMADGNEKKLEKVKAYREKIEKELETVCNDVLSLLDKFL

IKNCNDFQYESKVFYLKMKGDYYRYLAEVASGEKKNSVVEASEAAYKEAFEISK

EQMQPTHPIRLGLALNFSVFYYEIQNAPEQACLLAKQAFDDAIAELDTLNEDS

YKDSTLIMQLLRDNLTLWTSDQQDEEAGEGN