LCK(225-509)

Protein Information

Description

Truncated LCK (residues 225–509) fused to GST, AviTag,

 pyrophosphatase, and an N-terminal His8 tag.

Molecular Weight

63.47 kDa (uncut) / 33.25 (1st cut site)

pI

5.79 (uncut) / 5.32 (1st cut site)

Expression System

Tni

Buffer

50 mM Tris-HCl(pH 8.0 at 4C),0.15 M Sodium Chloride, 0.5 mM TCEP, 5% glycerol

Sequence

GPGSQTQKPQKPWWEDEWEVPRETLKLVERLGAGQFGE

VWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQ

HQRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLTINK

LLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIAD

FGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVW

SFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNC

PEELYQLMRLCWKERPEDRPTFDYLRSVLEDFFTATEGQYQ

PQP