Human KRAS4b

Protein Information

Description

A recombinant KRAS4b fusion protein with an N-terminal

 8xHis tag and MBP for enhanced solubility and purification,

 plus a TEV site for optional tag removal.

Molecular Weight

65.02 kDa

pI

5.72

Expression System

E. Coli

Buffer

50 mM HEPES (pH 7.5), 150 mM NaCl, 5 mM MgCl2, 1 mM DTT

Sequence

MGSSHHHHHHHHGSSMKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTG

IKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDK

AFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALD

KELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNA

GAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNID

TSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEG

LEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWY

AVRTAVINAASGRQTVDEALKDAQTGSSENLYFQGPGSMTEYKLVVVGAGGV

GKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRD

QYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPS

RTVDTKQAQDLARSYGIPFIETSAKTRQRVEDAFYTLVREIRQYRLKKISKEEKTP

GCVKIKKCIIM