Human 14-3-3β/α (3C-Cleaved)

Protein Information

Description

Recombinant dual-isoform 14-3-3ζ/δ protein with cleaved N-terminal tags

Molecular Weight

Un-cleaved: 58.79 kDa / Cleaved: 32.35 kDa

pI

Un-cleaved: 5.30 / Cleaved: 5.05

Expression System

BL21(DE3)

Buffer

20 mM HEPES (pH 8.0 at 4°C), 150 mM NaCl, 0.5 mM TCEP

Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFP

NLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGV

SRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDAL

DVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGG

GDHPPKGSSLEVLFQGPHHHHHHHHGSSGLNDIFEAQKIEWHEGSSENLYFQ

STMDKSELVQKAKLAEQAERYDDMAAAMKAVTEQGHELSNEERNLLSVAYK

NVVGARRSSWRVISSIEQKTERNEKKQQMGKEYREKIEAELQDICNDVLELLD

KYLIPNATQPESKVFYLKMKGDYFRYLSEVASGDNKQTTVSNSQQAYQEAFEIS

KKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLNEESYK

DSTLIMQLLRDNLTLWTSENQGDEGDAGEGEN