Human 14-3-3θ (3C Cleaved)

Protein Information

Description

Recombinant human 14-3-3θ protein with cleaved N-terminal tags

(GST, His8, Avi), processed with 3C protease.

Molecular Weight

58.48 kDa (un-cleaved) / 32.04 kDa (cleaved)

pI

5.25 (un-cleaved) / 4.98 (cleaved)

Expression System

BL21(DE3)

Buffer

20 mM HEPES (pH 8.0 at 4˚C), 150 mM NaCl, 0.5 mM TCEP

Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL

EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAV

LDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHV

THPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSK

YIAWPLQGWQATFGGGDHPPKGSSLEVLFQGPHHHHHHHHGSSGL

NDIFEAQKIEWHEGSSENLYFQSEKTELIQKAKLAEQAERYDDMATCM

KAVTEQGAELSNEERNLLSVAYKNVVGGRRSAWRVISSIEQKTDTSDKK

LQLIKDYREKVESELRSICTTVLELLDKYLIANATNPESKVFYLKMKGDYFR

YLAEVACGDDRKQTIDNSQGAYQEAFDISKKEMQPTHPIRLGLALNFSV

FYYEILNNPELACTLAKTAFDEAIAELDTLNEDSYKDSTLIMQLLRDNLTL

WTSDSAGEECDAAEGAEN