Human 14-3-3e (3C Cleaved)

Protein Information

Description

Recombinant 14-3-3ε isoform with cleaved N-terminal GST, His8, and Avi tags.

Molecular Weight

59.89 kDa (un-cleaved) / 33.45 kDa (cleaved)

pI

5.15 (un-cleaved) / 4.88 kDa (cleaved)

Expression System

BL21(DE3)

Buffer

20 mM HEPES (pH 8.0 at 4˚C), 150 mM NaCl, 0.5 mM TCEP

Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL

EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL

DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH

PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAW

PLQGWQATFGGGDHPPKGSSLEVLFQGPHHHHHHHHGSSGLNDIFEAQ

KIEWHEGSSENLYFQSDDREDLVYQAKLAEQAERYDEMVESMKKVAGMD

VELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQ

MVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGN

DRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACR

LAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKE

ALQDVEDENQ