Human 14-3-3σ (3C Cleaved)

Protein Information

Description

14-3-3σ isoform purified post 3C cleavage of N-terminal GST, His8, and Avi tags.

Molecular Weight

Un-cleaved: 58.49 kDa / Cleaved: 32.04 kDa

pI

Un-cleaved: 5.19 / Cleaved: 4.94

Expression System

BL21(DE3)

Buffer

20 mM Tris-HCl (pH 8.0 at 4°C), 150 mM NaCl

Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEF

PNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY

GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFML

YDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGW

QATFGGGDHPPKGSSLEVLFQGPHHHHHHHHGSSGLNDIFEAQKIEWHE

GSSENLYFQSERASLIQKAKLAEQAERYEDMAAFMKGAVEKGEELSCEERN

LLSVAYKNVVGGQRAAWRVLSSIEQKSNEEGSEEKGPEVREYREKVETELQ

GVCDTVLGLLDSHLIKEAGDAESRVFYLKMKGDYYRYLAEVATGDDKKRIID

SARSAYQEAMDISKKEMPPTNPIRLGLALNFSVFHYEIANSPEEAISLAKTTF

DEAMADLHTLSEDSYKDSTLIMQLLRDNLTLWTADNAGEEGGEAPQEPQS