Human 14-3-3y (3C Cleaved)

Protein Information

Description

Recombinant human 14-3-3γ protein with cleaved N-terminal

 fusion tags (GST, His8, Avi), leaving functional protein for interaction

 and signaling studies.

Molecular Weight

59.02 kDa (un-cleaved) / 32.58 kDa (cleaved)

pI

5.33 (un-cleaved) / 5.09 (cleaved)

Expression System

BL21(DE3)

Buffer

20 mM HEPES (pH 8.0 at 4˚C), 150 mM NaCl, 0.5 mM TCEP

Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFE

LGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISM

LEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTY

LNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQ

IDKYLKSSKYIAWPLQGWQATFGGGDHPPKGSSLEVLFQGPHHHHH

HHHGSSGLNDIFEAQKIEWHEGSSENLYFQSVDREQLVQKARLAEQA

ERYDDMAAAMKNVTELNEPLSNEERNLLSVAYKNVVGARRSSWRV

ISSIEQKTSADGNEKKIEMVRAYREKIEKELEAVCQDVLSLLDNYLIKNC

SETQYESKVFYLKMKGDYYRYLAEVATGEKRATVVESSEKAYSEAHEIS

KEHMQPTHPIRLGLALNYSVFYYEIQNAPEQACHLAKTAFDDAIAELD

TLNEDSYKDSTLIMQLLRDNLTLWTSDQQDDDGGEGNN