Human 14-3-3ζ/δ (3C Cleaved)

Protein Information

Description

Recombinant dual-isoform 14-3-3ζ/δ protein with cleaved N-terminal tags.

Molecular Weight

58.46 kDa (un-cleaved) / 32.02 kDa (cleaved)

pI

5.27 (un-cleaved) / 5.01 (cleaved)

Expression System

BL21(DE3)

Buffer

20 mM HEPES (pH 8.0 at 4˚C), 150 mM NaCl, 0.5 mM TCEP

Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLE

FPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDI

RYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPD

FMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPL

QGWQATFGGGDHPPKGSSLEVLFQGPHHHHHHHHGSSGLNDIFEAQK

IEWHEGSSENLYFQSDKNELVQKAKLAEQAERYDDMAACMKSVTEQGA

ELSNEERNLLSVAYKNVVGARRSSWRVVSSIEQKTEGAEKKQQMAREYRE

KIETELRDICNDVLSLLEKFLIPNASQAESKVFYLKMKGDYYRYLAEVAAGDD

KKGIVDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKAC

SLAKTAFDEAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDTQGDEAEAGE

GGEN