TEV (S219P)

Protein Information

Description 

A recombinant TEV protease variant (S219P mutation)

 fused to MBP and His tags for improved solubility and

 purification. Dual TEV sites enable flexible tag removal.

Molecular Weight

28.5

pI

9.75

Expression System

E. Coli

Buffer

50 mM Tris pH 8, 150 mM NaCl, 2 mM DTT

Sequence

HHHHHHHGKSLFKGPRDYNPISSTICHLTNESDGHTTSLYGIG

FGPFIITNKHLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGR

DMIIIRMPKDFPPFPQKLKFREPQREERICLVTTNFQTKSMSSM

VSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFIVGIHS

ASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSV

LWGGHKVFMPKPEEPFQPVKEATQLMNRRRRR