Human PP1C

Protein Information

Description

Human PP1C protein expressed in E. coli with an N-terminal His8 tag,

 GSSG linker, and TEV site in a pET-28a(+) vector.

Molecular Weight

39.44 kDa

pI

6.4

Expression System

BL21(DE3)

Buffer

50 mM HEPES (pH 7.5), 300 mM NaCl, 1 mM TCEP, 5 mM MnCl2

Sequence

MHHHHHHHHGSSGENLYFQGSMADLDKLNIDSIIQRLLEVRGSKPGKNV

QLQENEIRGLCLKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPP

ESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYD

ECKRRYNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIM

RPTDVPDQGLLCDLLWSDPDKDVLGWGENDRGVSFTFGAEVVAKFLHKHD

LDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDETLMC

SFQILKPAEKKKPNATRPVTPPRGMITKQAKK