CRBN

Protein Information

Description

A multi-tagged recombinant construct encoding

 the CRBN TBD (thalidomide-binding domain) fused

 to GST, PPase, and His tags in a pET28a(+) backbone.

Molecular Weight

40.73 kDa

pI

7.26

Expression System

BL21 (DE3)

Buffer

50 mM HEPES pH 7.4, 200 mM NaCl, 0.1 mM TCEP

Sequence

MGSSHHHHHHHHGSSMSPILGYWKIKGLVQPTRLLLE

YLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDG

DVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAV

LDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCH

KTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKL

VCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDH

PPKGSSLEVLFQGPLGSCTSLCCKQCQETEITTKNEIFSLSL

CGPMAAYVNPHGYVHETLTVYKASNLNLIGRPSTEHSWF

PGYAWTVAQCKICASHIGWKFTATKKDMSPQKFWGLTR

SALLPTI