Human PLK1 (WT)

Protein Information

Description

Recombinant wild-type human PLK1 with N-terminal

 His8 and AviTag, expressed in E. coli using the pET28a

vector.

Molecular Weight

71.6 kDa

pI

8.93

Expression System

BL21(DE3)

Buffer

20 mM Tris-HCl(pH 7.5 at 4°C),0.15 M Sodium Chloride, 0.5 mM TCEP 

Sequence

MHHHHHHHHGSSGLNDIFEAQKIEWHEGSSSAAVTAGKLARA

PADPGKAGVPGVAAPGAPAAAPPAKEIPEVLVDPRSRRRYVRG

RFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSME

ISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKA

LTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGD

FGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIM

YTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDP

TARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLT

VLNKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNA

SKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSV

GVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLK

YFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSN

GSVQINFFQDHTKLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCK

ELASRLRYARTMVDKLLSSRSASNRLKAS