WRN (517-1093)

Protein Information

Description

C-terminal WRN fragment (residues 517–1093) with a TEV cleavage site

 and C-terminal His6 tag.

Molecular Weight

66.8 kDa (Cleaved)

pI

8.48

Expression System

Tni

Buffer

50 mM HEPES (pH 7.5; 4˚C), 300 mM NaCl, 0.5 mM TCEP

Sequence

MNEGEEDDDKDFLWPAPNEEQVTCLKMYFGHSSFKPVQWKVIHSVLEERR

DNVAVMATGYGKSLCFQYPPVYVGKIGLVISPLISLMEDQVLQLKMSNIPAC

FLGSAQSENVLTDIKLGKYRIVYVTPEYCSGNMGLLQQLEADIGITLIAVDEAH

CISEWGHDFRDSFRKLGSLKTALPMVPIVALTATASSSIREDIVRCLNLRNPQIT

CTGFDRPNLYLEVRRKTGNILQDLQPFLVKTSSHWEFEGPTIIYCPSRKMTQQ

VTGELRKLNLSCGTYHAGMSFSTRKDIHHRFVRDEIQCVIATIAFGMGINKADI

RQVIHYGAPKDMESYYQEIGRAGRDGLQSSCHVLWAPADINLNRHLLTEIRNE

KFRLYKLKMMAKMEKYLHSSRCRRQIILSHFEDKQVQKASLGIMGTEKCCDN

CRSRLDHCYSMDDSEDTSWDFGPQAFKLLSAVDILGEKFGIGLPILFLRGSNSQ

RLADQYRRHSLFGTGKDQTESWWKAFSRQLITEGFLVEVSRYNKFMKICALTK

KGRNWLHKANTESQSLILQANEELCPKKFLLPSSKTVSSGTKEHCYNQAENLY

FQG